Cellular uptake of polymeric nanoparticles by bovine cumulus-oocyte complexes and their effect on in vitro developmental competence
نویسندگان
چکیده
Polymeric nanoparticles (NPs) are produced using bio-compatible and bio-degradable materials such as PLGA (Poly(lactic-co-glycolic acid)). This technology provides a valuable tool to deliver molecules the subcellular level with relatively low risk of cytotoxicity. However their use in field reproductive biotechnology is not yet scientifically substantiated. The aim present study was test if NPs can be taken-up by cumulus-enclosed oocytes first step towards potential oocyte-targeted applications enhance oocyte quality fertility. We conducted series experiments bovine vitro maturation model FITC-conjugated internalization (using laser-scanning confocal microscopy) effect some important physical (particle size) chemical (conjugation PEG) modifications. show evidence that cumulus cells less extent enclosed regardless NP size. transfer appear transcellular (via transzonal projections) paracellular zona pellucida). were detected vicinity quick 2 h post-exposure protein-free medium did compromise cell viability nor subsequent early embryo development or quality. These results suggest may have promising carriers for drug molecule delivery targeting oocytes.
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ژورنال
عنوان ژورنال: European Journal of Pharmaceutics and Biopharmaceutics
سال: 2021
ISSN: ['0939-6411', '1873-3441']
DOI: https://doi.org/10.1016/j.ejpb.2020.11.011